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KMID : 0895820070170020039
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Journal of Oriental Rehabilitation Medicine
2007 Volume.17 No. 2 p.39 ~ p.53
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A Study on the Effect of Gwanjul-bang(Guanjie-fang)-3 in Rheumatoid Arthritis
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Kim Hyun-Soo
Oh Min-Seok
Yoon Il-Ji
Heo Dong-Seok
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Abstract
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Objectives :Gwanjul-bang(Guanjie-fang)-3 (GJB-3) is a prescription used clinically to treat rheumatoid arthritis. The purpose of this study is to investigate the immunity responses of GJB-3 to the human fibroblast-like synoviocytes isolated from patients with rheumatoid arthritis.
Methods :Experimental groups were divided into 6 groups ; The hFLS were stimulated with hrTNF-α(100 ng/ml) and hrIL-1β(10 U/ml). Afterwards, They were treated with medium (CT), methotrexate (10㎍/ml, MTX) and GJB-3 (1, 10, 100㎍/ml) for 6 hours. The cells were harvested following the further incubation with [3H]-thymidine reagent for 4 hours. The WT group was cultured without cytokine stimulation.
Results :1. The proliferation of hFLSs was significantly reduced in 100㎍/ml dose. 2. The mRNA expression of TNF-α and the production of ROS was dose-dependently reduced in hFLS. 3. The mRNA expression of IL-1β, IL-6, IL-8, ICAM-1, NOS-Ⅱ, MMP-3 were reduced in high concentration. 4. The production of NO was significantly reduced in over 10㎍/ml dose. 5. The mRNA expression of TIMP-1 was dose-dependently increased.
Conclusion :We found out that GJB-3 is considered to be effective in treating rheumatoid arthritis.
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KEYWORD
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Gwanjul-bnag(Gunajie-fang)-3 (GJB-3), hFLSs, Rheumatoid arthritis
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